Anticorps Polyclonal de lapin anti-Draxin

Draxin Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 26342-1-AP

Synonymes

C1orf187, AGPA3119, Dorsal inhibitory axon guidance protein, Neucrin, PSEC0258

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 26342-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules SH-SY5Y, tissu de cervelet de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

26342-1-AP cible Draxin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Draxin Protéine recombinante Ag23666
• Nom complet: chromosome 1 open reading frame 187
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC021286
• Symbole du gène: C1orf187
• Identification du gène (NCBI): 374946
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C1orf187, also named Draxin(Dorsal repulsive axon guidance protein), is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).

Publications

Species	Application	Title
human	WB	Front Immunol; Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas; Authors - Jia-hao Bao
human	WB	Chem Biol Interact; Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes; Authors - Barbara Łasut-Szyszka

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