Anticorps Polyclonal de lapin anti-TMEM230

TMEM230 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21466-1-AP

Synonymes

C20orf30, HSPC274, TMEM230, UNQ2432/PRO4992

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Galerie de données de validation

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  WB analysis of HepG2 using 21466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21466-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21466-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21466-1-AP (TMEM230 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21466-1-AP (TMEM230 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21466-1-AP (TMEM230 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 21466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21466-1-AP (TMEM230 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21466-1-AP cible TMEM230 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM230 Protéine recombinante Ag15080
• Nom complet: chromosome 20 open reading frame 30
• Masse moléculaire calculée: 183 aa, 20 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC011990
• Symbole du gène: C20orf30
• Identification du gène (NCBI): 29058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).

Publications

Species	Application	Title
human	WB	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB,IHC	J Alzheimers Dis; TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease; Authors - Sandra L Siedlak
mouse	WB,IF	Exp Anim; TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models; Authors - Haiqing Zhang

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