Anticorps Polyclonal de lapin anti-C22orf25

• Phare
• Validé par KD/KO

C22orf25 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 27846-1-AP

Synonymes

C22orf25, TANGO2, Transport and Golgi organization protein 2 homolog

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Galerie de données de validation

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  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

27846-1-AP cible C22orf25 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C22orf25 Protéine recombinante Ag27127
• Nom complet: chromosome 22 open reading frame 25
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC041339
• Symbole du gène: C22orf25
• Identification du gène (NCBI): 128989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; The phenotype associated with variants in TANGO2 may be explained by a dual role of the protein in ER-to-Golgi transport and at the mitochondria.; Authors - Miroslav P Milev ; KD Validated
human	WB	J Inherit Metab Dis; Clinical and biological characterization of 20 patients with TANGO2 deficiency indicates novel triggers of metabolic crises and no primary energetic defect.; Authors - Claire-Marine Bérat
human,mouse	WB	Nature; HRG-9 homologues regulate haem trafficking from haem-enriched compartments; Authors - Fengxiu Sun ; KO Validated
human	WB,IF	Dis Model Mech; Intrinsic and extrinsic regulation of rhabdomyolysis susceptibility by Tango2; Authors - Euri S Kim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Antibody works well ( band near 45-50 kDa)

• Applications: Western Blot