Anticorps Polyclonal de lapin anti-RTCB-Specific

• Phare
• Validé par KD/KO

RTCB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 19809-1-AP

Synonymes

HSPC117, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8

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Galerie de données de validation

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  WB analysis using 19809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19809-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19809-1-AP

  IP result of anti-RtcB-Specific (IP:19809-1-AP, 3ug; Detection:19809-1-AP 1:500) with HeLa cells lysate 3000ug.

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  IP experiment of HeLa using 19809-1-AP

  IP result of anti-RtcB-Specific (IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.

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  IHC staining of human lung cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19809-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RTCB-Specific antibody (19809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 19809-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RTCB-Specific antibody (19809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 19809-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of A431 using 19809-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 2 publications below

Informations sur le produit

19809-1-AP cible RTCB-Specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTCB-Specific Protéine recombinante Ag13836
• Nom complet: chromosome 22 open reading frame 28
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000151
• Symbole du gène: RTCB
• Identification du gène (NCBI): 51493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RTCB (also known as HSPC117, C22orf28, FAAP, and D10Wsu52e) is a essential subunit of the tRNA ligase complex, which is localized in both the cytoplasm and nucleus, and is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB also serves as a cell adhesion protein and plays an important role in embryo and placenta development. Recently, RTCB has been identified as an UPR RNA ligase that catalyzes unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Publications

Species	Application	Title
human	WB	Cell; ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.; Authors - Yichen Xu
human	WB,IP	Mol Cell; A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.; Authors - Yanyan Lu
pig	WB	Nat Commun; Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.; Authors - Carmen Aguilar
human	WB	J Biol Chem; The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.; Authors - Irem Unlu ; KO Validated
human	IP	Life Sci Alliance; Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.; Authors - Alexandra Papaioannou
human	WB	J Immunol; The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1; Authors - Lei Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Megan (Verified Customer) (08-07-2025)

I used this antibody to verify a knockdown of RtcB in Hek293T cells and it worked well. Gave a specific band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Ilham (Verified Customer) (08-06-2024)

It works very well - the AB was diluted 1:2000 in 5% BSA -chemiluminescence blots using the ChemiDoc Imaging System.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

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