Anticorps Polyclonal de lapin anti-RtcB-Specific

• Phare
• Validé par KD/KO

RtcB-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19809-1-AP

Synonymes

C22orf28, FAAP, focal adhesion associated protein, hematopoietic stem/progenitor cell 117, HSPC117, RtcB, RtcB-Specific

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Galerie de données de validation

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  WB analysis using 19809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19809-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19809-1-AP

  IP Result of anti-RtcB-Specific (IP:19809-1-AP, 3ug; Detection:19809-1-AP 1:500) with HeLa cells lysate 3000ug.

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  IP experiment of HeLa using 19809-1-AP

  IP result of anti-RtcB-Specific(IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.

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  IHC staining of human lung cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19809-1-AP

  Immunofluorescent analysis of HeLa cells using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 2 publications below

Informations sur le produit

19809-1-AP cible RtcB-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RtcB-Specific Protéine recombinante Ag13836
• Nom complet: chromosome 22 open reading frame 28
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000151
• Symbole du gène: C22orf28
• Identification du gène (NCBI): 51493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Publications

Species	Application	Title
human	WB	Cell; ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.; Authors - Yichen Xu
human	WB,IP	Mol Cell; A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.; Authors - Yanyan Lu
pig	WB	Nat Commun; Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.; Authors - Carmen Aguilar
human	WB	J Biol Chem; The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.; Authors - Irem Unlu ; KO Validated
human	IP	Life Sci Alliance; Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.; Authors - Alexandra Papaioannou
human	WB	J Immunol; The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1; Authors - Lei Cao