Anticorps Polyclonal de lapin anti-ABHD18

ABHD18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 25036-1-AP

Synonymes

C4orf29, Abhydrolase domain-containing protein 18, Alpha/beta hydrolase domain-containing protein 18, Protein ABHD18

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Galerie de données de validation

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  WB analysis of PC-3 using 25036-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25036-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25036-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 25036-1-AP

  Immunofluorescent analysis of A549 cells using 25036-1-AP (C4orf29 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 25036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using C4orf29 antibody (25036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A549, tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25036-1-AP cible ABHD18 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABHD18 Protéine recombinante Ag19120
• Nom complet: chromosome 4 open reading frame 29
• Masse moléculaire calculée: 414 aa, 47 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC128143
• Symbole du gène: C4orf29
• Identification du gène (NCBI): 80167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABHD18 (α/β hydrolase domain-containing protein 18) is a transmembrane protein in mammals whose function has not yet been fully elucidated. It belongs to the large α/β hydrolase superfamily, whose members typically possess hydrolase activity and are involved in a variety of metabolic reactions. The ABHD18 protein is localized on the lysosomal membrane and may participate in intracellular lipid metabolism or signal transduction processes. Recent studies have shown that ABHD18 is the key enzyme in human cells that catalyzes the deacylation of cardiolipin to MLCL. The loss of ABHD18 function can significantly alleviate mitochondrial supercomplex defects, energy metabolism disorders, and cardiomyopathy phenotypes caused by TAZ deficiency, even achieving a "genetic suppression" effect in mice and patient cells. This not only reveals ABHD18 as a core node in the cardiolipin remodeling pathway but also provides an entirely new therapeutic approach for mitochondrial diseases such as Barth syndrome.

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