Anticorps Polyclonal de lapin anti-Complement C6

Complement C6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 17239-1-AP

Synonymes

C6, complement component 6, Complement component C6

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Galerie de données de validation

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  WB analysis of human blood using 17239-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 17239-1-AP

  IP result of anti-Complement C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:3000) with human plasma lysate 3200 ug.

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: sang humain,
• Résultats positifs en IP: plasma humain,
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

17239-1-AP cible Complement C6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Complement C6 Protéine recombinante Ag11083
• Nom complet: complement component 6
• Masse moléculaire calculée: 934 aa, 105 kDa
• Poids moléculaire observé: 105-120 kDa
• Numéro d’acquisition GenBank: BC035723
• Symbole du gène: C6
• Identification du gène (NCBI): 729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

Publications

Species	Application	Title
human	IF	PLoS One; Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	WB	Oral Dis; Analysis of Salivary Exosomal Proteins in Young Adults with Severe Periodontitis.; Authors - Xiaoyi Huang
human	IHC	Ann Diagn Pathol; The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder; Authors - Gerard J. Nuovo
rat	IF	Ren Fail; Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	IHC	Ann Diagn Pathol; The amplification of CNS damage in Alzheimer's disease due to SARS-CoV2 infection; Authors - Gerard J. Nuovo
human	IF	Nat Commun; A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells; Authors - Xue Li

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