Anticorps Polyclonal de lapin anti-C7orf47

C7orf47 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 24214-1-AP

Synonymes

Protein phosphatase 1 regulatory subunit 35, UPF0683 protein C7orf47

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Galerie de données de validation

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

24214-1-AP cible C7orf47 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C7orf47 Protéine recombinante Ag18340
• Nom complet: chromosome 7 open reading frame 47
• Masse moléculaire calculée: 253 aa, 28 kDa
• Numéro d’acquisition GenBank: BC026269
• Symbole du gène: C7orf47
• Identification du gène (NCBI): 221908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Elife; PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.; Authors - Andrew Michael Sydor

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (04-24-2023)

Weak centriolar staining. While this antibody works well for Westernblotting, the expected centriolar staining in IF is rather weak. Yet, this antibody works under the following conditions: RPE1 cells stained for Hoechst (DNA marker, in green), PPP1r35 (centriolar marker, in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

C7orf47 Antibody Immunofluorescence validation (1:50 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24214-1-AP)

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