Anticorps Polyclonal de lapin anti-CA11

CA11 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15435-1-AP

Synonymes

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

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Galerie de données de validation

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  WB analysis of human kidney using 15435-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15435-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal humain
• Résultats positifs en IHC: tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15435-1-AP cible CA11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA11 Protéine recombinante Ag7712
• Nom complet: carbonic anhydrase XI
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kda, 45 kDa
• Numéro d’acquisition GenBank: BC002662
• Symbole du gène: CA11
• Identification du gène (NCBI): 770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).