Anticorps Polyclonal de lapin anti-CA12

• Phare
• Validé par KD/KO

CA12 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 15180-1-AP

Synonymes

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3

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Galerie de données de validation

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  WB analysis using 15180-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected MCF-7 cells.

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  WB analysis of A549 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected A549 cells.

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  WB analysis of PC-3 using 15180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15180-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HEK-293 using 15180-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug CA12 antibody (15180-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules MCF-7
• Résultats positifs en IHC: tissu pancréatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

15180-1-AP cible CA12 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA12 Protéine recombinante Ag7107
• Nom complet: carbonic anhydrase XII
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 44 kDa, 75-80 kDa
• Numéro d’acquisition GenBank: BC000278
• Symbole du gène: CA12
• Identification du gène (NCBI): 771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Publications

Species	Application	Title
human	WB, IF	J Exp Med; Large pH oscillations promote host defense against human airways infection.; Authors - Dusik Kim
human	WB	Mol Oncol; Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.; Authors - Maria B Asparuhova
human	WB,IF	Hum Mol Genet; Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.; Authors - Melissa Lee
human	IHC	Int J Mol Sci; Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.; Authors - Zihe Huo
human	WB,IF	J Enzyme Inhib Med Chem; Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.; Authors - Soyoung Hwang
human	WB	Front Oncol; Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.; Authors - FeiYan Ai