Anticorps Polyclonal de lapin anti-CACNA1S

CACNA1S Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 22279-1-AP

Synonymes

CACH1, CACN1, CACNL1A3, Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Cav1.1

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Galerie de données de validation

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  WB analysis using 22279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

22279-1-AP cible CACNA1S dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: calcium channel, voltage-dependent, L type, alpha 1S subunit
• Masse moléculaire calculée: 212 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: NM_000069
• Symbole du gène: CACNA1S
• Identification du gène (NCBI): 779
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.

Publications

Species	Application	Title
human	IHC	Reproduction; Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.; Authors - Ru Zheng
human	WB	bioRxiv; Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias

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