Anticorps Polyclonal de lapin anti-CALCOCO1

• Phare
• Validé par KD/KO

CALCOCO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 19843-1-AP

Synonymes

Calcium-binding and coiled-coil domain-containing protein 1, Calphoglin, Cocoa, Coiled-coil coactivator protein, PP13275

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Galerie de données de validation

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  WB analysis using 19843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALCOCO1 antibody (19843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HT-1080, cellules MCF-7, cellules NIH/3T3, cellules Ramos
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de souris, tissu rénal humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

19843-1-AP cible CALCOCO1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CALCOCO1 Protéine recombinante Ag13876
• Nom complet: calcium binding and coiled-coil domain 1
• Masse moléculaire calculée: 691 aa, 77 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC003177
• Symbole du gène: CALCOCO1
• Identification du gène (NCBI): 57658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Elife; Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.; Authors - Junghyun Lim ; KO Validated
mouse	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper
mouse	WB	Nat Commun; Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer; Authors - Lucia Taraborrelli

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