Anticorps Polyclonal de lapin anti-NDP52

• Phare
• Validé par KD/KO

NDP52 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

43

N° de cat : 12229-1-AP

Synonymes

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

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Galerie de données de validation

• 

  WB analysis of HeLa using 12229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 12229-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12229-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12229-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12229-1-AP

  IP Result of anti-NDP52 (IP:12229-1-AP, 3ug; Detection:12229-1-AP 1:1000) with HeLa cells lysate 2150ug.

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 12229-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12229-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NDP52 antibody (12229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu de muscle squelettique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

12229-1-AP cible NDP52 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDP52 Protéine recombinante Ag2866
• Nom complet: calcium binding and coiled-coil domain 2
• Masse moléculaire calculée: 446 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC015893
• Symbole du gène: CALCOCO2
• Identification du gène (NCBI): 10241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Publications

Species	Application	Title
mouse	WB	Cell; NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.; Authors - Zhenyu Zhong
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KO Validated
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
mouse	WB	Nat Commun; Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.; Authors - Insup Choi
mouse	WB	Redox Biol; A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease; Authors - Yi Sun

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