Anticorps Polyclonal de lapin anti-Caldesmon

• Phare
• Validé par KD/KO

Caldesmon Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 20887-1-AP

Synonymes

CALD1, CAD, caldesmon 1, CDM

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Galerie de données de validation

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  WB analysis using 20887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20887-1-AP

  WB result of Caldesmon antibody (20887-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caldesmon transfected HeLa cells.

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  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20887-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Caldesmon Polyclonal antibody (20887-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HeLa, cellules HT-1080, cellules MDA-MB-231, cellules PC-3, cellules SKOV-3
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

20887-1-AP cible Caldesmon dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caldesmon Protéine recombinante Ag13676
• Nom complet: caldesmon 1
• Masse moléculaire calculée: 538 aa, 63 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC040354
• Symbole du gène: CALD1
• Identification du gène (NCBI): 800
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

Publications

Species	Application	Title
human	WB,IF	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB,IF	Front Physiol; PMID: 30108509; Authors - Iddrisu B Yabasin
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
mouse		bioRxiv; IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells; Authors - Victoria A Wong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (01-22-2024)

Excellent antibody. Results are better than expected. Works well in Co-IP as well as in western blotting

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293, A7R5, HTR8, Liver, Placenta, Aorta

Caldesmon Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in HEK293, A7R5, HTR8, Liver, Placenta, Aorta (Cat no:20887-1-AP)

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