Anticorps Polyclonal de lapin anti-Caldesmon

• Phare
• Validé par KD/KO

Caldesmon Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 20887-1-AP

Synonymes

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

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Galerie de données de validation

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  WB analysis of HeLa using 20887-1-AP

  WB result of Caldesmon antibody (20887-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caldesmon transfected HeLa cells.

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  WB analysis using 20887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20887-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Caldesmon (20887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HeLa, cellules HT-1080, cellules MDA-MB-231, cellules PC-3, cellules SKOV-3
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20887-1-AP cible Caldesmon dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caldesmon Protéine recombinante Ag13676
• Nom complet: caldesmon 1
• Masse moléculaire calculée: 538 aa, 63 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC040354
• Symbole du gène: CALD1
• Identification du gène (NCBI): 800
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

Publications

Species	Application	Title
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB,IF	Front Physiol; PMID: 30108509; Authors - Iddrisu B Yabasin
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
mouse		bioRxiv; IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells; Authors - Victoria A Wong
human	WB	Cancers (Basel); AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma; Authors - Jiakang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gaurav (Verified Customer) (01-22-2024)

Excellent antibody. Results are better than expected. Works well in Co-IP as well as in western blotting

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293, A7R5, HTR8, Liver, Placenta, Aorta

Caldesmon Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in HEK293, A7R5, HTR8, Liver, Placenta, Aorta (Cat no:20887-1-AP)