Anticorps Polyclonal de lapin anti-CaMKII delta-Specific

• Phare
• Validé par KD/KO

CaMKII delta-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 20667-1-AP

Synonymes

CaM kinase II subunit delta, CaMK II subunit delta, CAMK2D, CAMKD, CaMKII delta-Specific

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Galerie de données de validation

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  WB analysis using 20667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20667-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20667-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 20667-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20667-1-AP

  IP Result of anti-CaMKII delta-Specific (IP:20667-1-AP, 4ug; Detection:20667-1-AP 1:600) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu cérébral de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20667-1-AP cible CaMKII delta-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: calcium/calmodulin-dependent protein kinase II delta
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_001221
• Symbole du gène: CAMK2D
• Identification du gène (NCBI): 817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.; Authors - Cui Ma
mouse	IHC	Cell Death Dis; miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.; Authors - Xudong Zhang
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang
rat	WB	J Pain Res; Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway.; Authors - Peng Chen
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu
mouse,rat	WB	Cell Mol Life Sci; JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes; Authors - Jiachen Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000) and IF (1:200)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200