Anticorps Polyclonal de lapin anti-CAMKK2

• Phare
• Validé par KD/KO

CAMKK2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

38

N° de cat : 11549-1-AP

Synonymes

CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2, CaM KK beta, CAMKK, CaMKK 2, CaMKK beta, CAMKK2, CAMKKB, KIAA0787

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Galerie de données de validation

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  WB analysis using 11549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11549-1-AP

  WB result of CAMKK2 antibody (11549-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMKK2 transfected Jurkat cells.

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  WB analysis of fetal human brain using 11549-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11549-1-AP

  IP result of anti-CAMKK2(IP:11549-1-AP, 4ug; Detection:11549-1-AP 1:500) with PC-3 cells lysate 960 ug.

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  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 11549-1-AP

  Immunofluorescent analysis of PC-3 cells using 11549-1-AP (CAMKK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu cérébral humain fœtal
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 35 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

11549-1-AP cible CAMKK2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAMKK2 Protéine recombinante Ag2093
• Nom complet: calcium/calmodulin-dependent protein kinase kinase 2, beta
• Masse moléculaire calculée: 588 aa, 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC026060
• Symbole du gène: CAMKK2
• Identification du gène (NCBI): 10645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.; Authors - Chen-Song Zhang
human	WB	Cancer Discov; Macropinocytosis in Cancer-Associated Fibroblasts Is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness.; Authors - Yijuan Zhang ; KD Validated
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB,IF	Autophagy; m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.; Authors - Yabing Chen