Anticorps Polyclonal de lapin anti-CAMSAP2

• Phare
• Validé par KD/KO

CAMSAP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

39

N° de cat : 17880-1-AP

Synonymes

CAMSAP1L1, CAMSAP2, KIAA1078

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Galerie de données de validation

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  WB analysis using 17880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17880-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 17880-1-AP

  IP Result of anti-CAMSAP2 (IP:17880-1-AP, 4ug; Detection:17880-1-AP 1:3000) with PC-3 cells lysate 5000ug.

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules PC-3, tissu cérébral de souris
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IF: See 29 publications below

Informations sur le produit

17880-1-AP cible CAMSAP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAMSAP2 Protéine recombinante Ag11794
• Nom complet: calmodulin regulated spectrin-associated protein 1-like 1
• Masse moléculaire calculée: 1478 aa, 167 kDa
• Poids moléculaire observé: 168 kDa
• Numéro d’acquisition GenBank: BC065508
• Symbole du gène: CAMSAP1L1
• Identification du gène (NCBI): 23271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMSAP2, also known as CAMSAP1L1, is a microtubule minus-end binding protein, and is required for proper microtubule organization in neurons. CAMSAP2 stabilizes noncentrosomal microtubules and is important for neuronal polarity, axon specification, and dendritic branch formation.

Publications

Species	Application	Title
human	IF	Nat Struct Mol Biol; A chemical genetics approach to examine the functions of AAA proteins.; Authors - Tommaso Cupido
rat	WB,IF	Neuron; Microtubule Minus-End Binding Protein CAMSAP2 Controls Axon Specification and Dendrite Development.; Authors - Kah Wai Yau ; KD Validated
mouse	IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
rat	IF	Neuron; TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.; Authors - Sam F B van Beuningen
human	WB	Neuron; Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development.; Authors - Naoki Nakagawa
mouse	IF	Neuron; Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment.; Authors - Marijn Kuijpers