Anticorps Polyclonal de lapin anti-CAP1

• Phare
• Validé par KD/KO

CAP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 16231-1-AP

Synonymes

Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

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Galerie de données de validation

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  WB analysis using 16231-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16231-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16231-1-AP

  IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug.

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules COLO 320, cellules HepG2
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

16231-1-AP cible CAP1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAP1 Protéine recombinante Ag8876
• Nom complet: CAP, adenylate cyclase-associated protein 1 (yeast)
• Masse moléculaire calculée: 475 aa, 52 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC013963
• Symbole du gène: CAP1
• Identification du gène (NCBI): 10487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

Publications

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; CAP1 binds and activates adenylyl cyclase in mammalian cells.; Authors - Xuefeng Zhang
human	WB,IF	J Biol Chem; Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.; Authors - Xuefeng Zhang ; KD Validated
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
bovine	WB	Cell Biochem Funct; Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.; Authors - Xiaohan Yuan ; KD Validated
mouse	WB	Cell Mol Life Sci; Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology; Authors - Anika Heinze

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kuldeep (Verified Customer) (09-17-2025)

sharp band, little over 50KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hippocamus homogenate

CAP1 Antibody Western Blot validation (1:1000 dilution) in Hippocamus homogenate (Cat no:16231-1-AP)

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