Anticorps Monoclonal anti-CAP1

• Phare
• Validé par KD/KO

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2H3A10

N° de cat : 68207-1-Ig

Synonymes

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

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Galerie de données de validation

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  WB analysis using 68207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 68207-1-Ig

  WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells.

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  WB analysis of pig lung using 68207-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68207-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 68207-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 68207-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 68207-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HepG2, tissu placentaire humain, tissu pulmonaire de porc, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu pulmonaire de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

68207-1-Ig cible CAP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAP1 Protéine recombinante Ag8876
• Nom complet: CAP, adenylate cyclase-associated protein 1 (yeast)
• Masse moléculaire calculée: 475 aa, 52 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC013963
• Symbole du gène: CAP1
• Identification du gène (NCBI): 10487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

Publications

Species	Application	Title
human		Int Immunopharmacol; Single-cell RNA sequencing reveals cell-cell communication and potential biomarker in sepsis and septic shock patients; Authors - Guilin Li
human	IF	Acta Neuropathol; Enhanced microglial dynamics and a paucity of tau seeding in the amyloid plaque microenvironment contribute to cognitive resilience in Alzheimer's disease; Authors - Nur Jury-Garfe
human	WB	J Neurochem; CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation; Authors - Jie Yang

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