Anticorps Polyclonal de lapin anti-CAPG

• Phare
• Validé par KD/KO

CAPG Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 10194-1-AP

Synonymes

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Galerie de données de validation

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  WB analysis using 10194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10194-1-AP

  WB result of CAPG antibody (10194-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

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  WB analysis of rat lung using 10194-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

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  IP experiment of mouse heart using 10194-1-AP

  IP Result of anti-CAPG (IP:10194-1-AP, 4ug; Detection:10194-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cœur de rat, cœur de souris, tissu pulmonaire de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

10194-1-AP cible CAPG dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPG Protéine recombinante Ag0248
• Nom complet: capping protein (actin filament), gelsolin-like
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: BC000728
• Symbole du gène: CAPG
• Identification du gène (NCBI): 822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.; Authors - Khositseth Sookkasem S
human	WB	J Proteome Res; Laser microdissection and two-dimensional difference gel electrophoresis reveal the role of a novel macrophage-capping protein in lymph node metastasis in gastric cancer.; Authors - Ichikawa Hiroshi H
human	WB	Clin Proteomics; Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.; Authors - Lavanya Balakrishnan
human	WB	Inflammation; PMID: 25586482; Authors - Xin Ma
human	WB	Am J Respir Cell Mol Biol; Up-regulation of two actin-associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia.; Authors - Zhang Ruifeng R ; KD Validated
human	WB	Proteomics; Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells.; Authors - Liao Shi-Hua SH