Anticorps Polyclonal de lapin anti-Calpain 1

• Phare
• Validé par KD/KO

Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

Avis (3)

N° de cat : 10538-1-AP

Synonymes

Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

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Galerie de données de validation

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  WB analysis of HeLa using 10538-1-AP

  WB result of Calpain 1 antibody (10538-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 1 transfected HeLa cells.

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  WB analysis using 10538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10538-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10538-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10538-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10538-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10538-1-AP

  IP Result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10538-1-AP

  Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 10538-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Calpain 1 antibody (10538-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A2780, cellules A431, cellules HepG2, cellules Jurkat, cellules K-562, cellules PC-3, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu cardiaque humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

10538-1-AP cible Calpain 1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 1 Protéine recombinante Ag0810
• Nom complet: calpain 1, (mu/I) large subunit
• Masse moléculaire calculée: 704 aa, 82 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC008751
• Symbole du gène: CAPN1
• Identification du gène (NCBI): 823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang
mouse	WB	Pharmacol Res; TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.; Authors - Wen Tian
mouse	WB	Int Immunopharmacol; The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice; Authors - Mengqing Liu
human	WB	J Biol Chem; Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.; Authors - Hui-Yan Lei
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; Elastic titin properties and protein quality control in the aging heart.; Authors - Senem Salcan
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.; Authors - Haipeng Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brice-Emmanuel (Verified Customer) (09-11-2023)

Works perfectly, I obtained a single strip of the desired size using the 1/5000 dilution in NFDM 5%.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Mouse Heart Tissue

Calpain 1 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:10538-1-AP)

FH

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cell overexpression

FH

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart