Anticorps Polyclonal de lapin anti-CAPRIN2

• Phare
• Validé par KD/KO

CAPRIN2 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF

Conjugaison

Non conjugué

Publications

2

Avis (2)

N° de cat : 20766-1-AP

Synonymes

C1QDC1, caprin 2, caprin family member 2, CAPRIN2, EEG 1, EEG1, KIAA1873, Protein EEG 1, RNA granule protein 140, RNG140

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Galerie de données de validation

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  WB analysis of Y79 using 20766-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20766-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of SH-SY5Y using 20766-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 20766-1-AP

  IP Result of anti-CAPRIN2 (IP:20766-1-AP, 4ug; Detection:20766-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IF Staining of HepG2 using 20766-1-AP

  Immunofluorescent analysis of HepG2 cells using 20766-1-AP (CAPRIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 20766-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPRIN2 antibody 20766-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HEK-293, cellules SH-SY5Y
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20766-1-AP cible CAPRIN2 dans les applications de WB, IP, IHC, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPRIN2 Protéine recombinante Ag14745
• Nom complet: caprin family member 2
• Masse moléculaire calculée: 1127 aa, 126 kDa
• Poids moléculaire observé: 126-150 kDa
• Numéro d’acquisition GenBank: BC007520
• Symbole du gène: CAPRIN2
• Identification du gène (NCBI): 65981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IHC	Neuroendocrinology; Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.; Authors - Soledad Bárez-López
mouse	WB, IF	Dev Dyn; Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.; Authors - Soma Dash ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (04-29-2019)

Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells, Rat neuronal samples

CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP)

FH

Criseyda (Verified Customer) (09-21-2018)

Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MRC-9 Lung Fiberblasts

CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP)