Anticorps Polyclonal de lapin anti-CARD14

CARD14 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10400-1-AP

Synonymes

BIMP2, CARD14, Carma 2, CARMA2

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Galerie de données de validation

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  WB analysis of HeLa using 10400-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10400-1-AP (CARD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

10400-1-AP cible CARD14 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CARD14 Protéine recombinante Ag0628
• Nom complet: caspase recruitment domain family, member 14
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 113-140 kDa
• Numéro d’acquisition GenBank: BC018142
• Symbole du gène: CARD14
• Identification du gène (NCBI): 79092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CARD14/CARMA2 is a novel caspase recruitment domain-containing protein 14 and is also a membrane-associated guanylate kinase (MAGUK) family member. CARD domain is a protein-binding element that mediates the assembly of CARD-containing proteins into apoptosis and NF-kappaB signaling complexes, which has been implicated in inflammatory disorders. CARD14 activates NF-kappaB signaling via interaction with BCL10. Defects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2), which is a common inflammatory disorder of the skin and other organs caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; MALT1 targeting suppresses CARD14-induced psoriatic dermatitis in mice.; Authors - Elien Van Nuffel
human	WB	Oncogene; MALT1 is a critical mediator of PAR1-driven NF-κB activation and metastasis in multiple tumor types.; Authors - J Randall McAuley
human	WB	Biomedicines; CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells; Authors - Domien Vanneste
mouse	WB	Biochem J; CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation; Authors - Paul A O'Sullivan

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