Anticorps Polyclonal de lapin anti-Caspase 4/P20/P10

• Phare
• Validé par KD/KO

Caspase 4/P20/P10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 11856-1-AP

Synonymes

CASP4, Caspase4, CASP 4, CASP-4, Caspase 4

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Galerie de données de validation

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  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/P20/P10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/P20/P10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/P20/P10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/P20/P10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11856-1-AP (Caspase 4/P20/P10 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11856-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 4/P20/P10 antibody (11856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11856-1-AP cible Caspase 4/P20/P10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 4/P20/P10 Protéine recombinante Ag2429
• Nom complet: caspase 4, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 377 aa, 43 kDa
• Poids moléculaire observé: 43-48 kDa
• Numéro d’acquisition GenBank: BC017839
• Symbole du gène: Caspase 4
• Identification du gène (NCBI): 837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Publications

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB,IF	Arch Biochem Biophys; GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis; Authors - Xiaoping Xie
human	WB	Oral Dis; Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway; Authors - Bing-Jing Sun
		J Diabetes Investig; VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation.; Authors - Si Wen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shan (Verified Customer) (07-10-2025)

The antibody works good when tested by western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hepatocytes

Caspase 4/P20/P10 Antibody Western Blot validation (1:1000 dilution) in hepatocytes (Cat no:11856-1-AP)

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