Anticorps Polyclonal de lapin anti-Catalase

• Phare
• Validé par KD/KO

Catalase Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

128

Avis (4)

N° de cat : 21260-1-AP

Synonymes

CAT, catalase, Cell and organelle markers, Peroxisome Marker

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Galerie de données de validation

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  WB analysis using 21260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21260-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21260-1-AP

  WB result of Catalase antibody (21260-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells.

• 

  WB analysis of Raji using 21260-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21260-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21260-1-AP (CAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21260-1-AP

  Immunofluorescent analysis of HepG2 cells using 21260-1-AP (Catalase antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 21260-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Catalase antibody (21260-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02, cellules Raji, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 121 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Informations sur le produit

21260-1-AP cible Catalase dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, souris, Meretrix meretrix, Champignon, Chauve-souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Catalase Protéine recombinante Ag15383
• Nom complet: catalase
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC112219
• Symbole du gène: CAT
• Identification du gène (NCBI): 847
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
mouse	WB	Nat Commun; Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity; Authors - Jinjie Duan
mouse	WB	Nat Commun; Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.; Authors - Jun Zhou
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (08-11-2022)

It worked well for western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human fibroblasts

FH

Maximilian (Verified Customer) (06-03-2022)

The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Maximilian (Verified Customer) (05-27-2022)

Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Tom (Verified Customer) (08-25-2021)

Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

Catalase Antibody Western Blot validation (1:2000 dilution) in (Cat no:21260-1-AP)

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