Anticorps Polyclonal de lapin anti-Caveolin-1

• Phare
• Validé par KD/KO

Caveolin-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

81

Avis (7)

N° de cat : 16447-1-AP

Synonymes

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21

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Galerie de données de validation

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  WB analysis using 16447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16447-1-AP (Caveolin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16447-1-AP

  WB result of Caveolin-1 antibody (16447-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells.

• 

  IP experiment of A549 using 16447-1-AP

  IP Result of anti-Caveolin-1 (IP:16447-1-AP, 3ug; Detection:16447-1-AP 1:500) with A549 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16447-1-AP (CAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque humain, tissu de cancer du poumon humain, tissu hépatique humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 67 publications below
• IHC: See 13 publications below
• IF: See 18 publications below
• IP: See 4 publications below

Informations sur le produit

16447-1-AP cible Caveolin-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caveolin-1 Protéine recombinante Ag8049
• Nom complet: caveolin 1, caveolae protein, 22kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC006432
• Symbole du gène: CAV1
• Identification du gène (NCBI): 857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	WB	Nucleic Acids Res; Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.; Authors - Huawei Wang
mouse	WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	IF	Small; Smart Carbon Nanotubes with Laser-Controlled Behavior in Gene Delivery and Therapy through a Non-Digestive Trafficking Pathway.; Authors - Fenfen Kong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (01-20-2024)

Excellent antibody for use by western blotting. Diluted in 3% BSA PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

Caveolin-1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:16447-1-AP)

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Emma (Verified Customer) (03-15-2022)

Works really well @ 1:1000 for WB in PC3 and DU145 cells. Single band seen.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

FH

Kushal (Verified Customer) (08-09-2021)

We tried to use the antibody at high concentrations of 1:100, yet we do not get bands in our blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Caco-2

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