Anticorps Polyclonal de lapin anti-c-Cbl

• Phare
• Validé par KD/KO

c-Cbl Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 25818-1-AP

Synonymes

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

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Galerie de données de validation

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  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25818-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25818-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

• 

  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Jurkat, cellules Raji, cellules Ramos, cellules Romas
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de lymphome humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

25818-1-AP cible c-Cbl dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-Cbl Protéine recombinante Ag23033
• Nom complet: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Masse moléculaire calculée: 906 aa, 100 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC132733
• Symbole du gène: CBL
• Identification du gène (NCBI): 867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Publications

Species	Application	Title
human	WB,IF	Hepatology; PMID: 29729186; Authors - Huixia Zhang ; KD Validated
human	WB,IF,CoIP	Cell Rep; Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis; Authors - Huan Li ; KD Validated
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Cell Commun Signal; Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells; Authors - Limei Xu
human	WB	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan
human	WB	J Control Release; Imatinib co-loaded targeted realgar nanocrystal for synergistic therapy of chronic myeloid leukemia.; Authors - Shengmei Wang