Anticorps Polyclonal de lapin anti-CBS

• Phare
• Validé par KD/KO

CBS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

96

N° de cat : 14787-1-AP

Synonymes

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase

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Galerie de données de validation

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  WB analysis of HEK-293 using 14787-1-AP

  WB result of CBS antibody (14787-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected HEK-293 cells.

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  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14787-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14787-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14787-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 14787-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14787-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14787-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14787-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14787-1-AP

  IP Result of anti-CBS (IP:14787-1-AP, 3ug; Detection:14787-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 14787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 14787-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules LNCaP, cellules MCF-7, cellules SKOV-3, cellules THP-1, tissu cérébral de rat, tissu cérébral de souris, tissu de côlon de souris, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 86 publications below
• IHC: See 14 publications below
• IF: See 15 publications below

Informations sur le produit

14787-1-AP cible CBS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBS Protéine recombinante Ag6437
• Nom complet: cystathionine-beta-synthase
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61-63 kDa
• Numéro d’acquisition GenBank: BC000440
• Symbole du gène: CBS
• Identification du gène (NCBI): 875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Publications

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
canine	IF	Nat Commun; Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation.; Authors - Yuhki Koike
mouse	WB	Redox Biol; Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasis.; Authors - Rachael Bok
human	WB	Redox Biol; Deletion of cystathionine-γ-lyase in bone marrow-derived cells promotes colitis-associated carcinogenesis.; Authors - Ketan K Thanki
mouse	WB,IF	Redox Biol; Endogenous H2S targets mitochondria to promote continual phagocytosis of erythrocytes by microglia after intracerebral hemorrhage; Authors - Xiaoling Yan

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