Anticorps Polyclonal de lapin anti-CBX1

• Phare
• Validé par KD/KO

CBX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10241-2-AP

Synonymes

CBX, CBX1, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 BETA, HP1Hs beta, HP1Hsbeta, M31, MOD1, Modifier 1 protein, p25beta

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Galerie de données de validation

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  WB analysis using 10241-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10241-2-AP

  WB result of CBX1 antibody (10241-2-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells.

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  WB analysis of human brain using 10241-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10241-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10241-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10241-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10241-2-AP

  IP Result of anti-CBX1 (IP:10241-2-AP, 3ug; Detection:10241-2-AP 1:500) with HEK-293 cells lysate 1480ug.

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  IHC staining of mouse testis using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10241-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10241-2-AP (CBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules MCF-7, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

10241-2-AP cible CBX1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX1 Protéine recombinante Ag0328
• Nom complet: chromobox homolog 1 (HP1 beta homolog Drosophila )
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC002609
• Symbole du gène: CBX1
• Identification du gène (NCBI): 10951
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Publications

Species	Application	Title
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB,IHC,IF	J Cell Biol; Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.; Authors - Jiang Wei-Qin WQ ; KD Validated
human	WB	J Virol; PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.; Authors - Zhangping He
human	WB,IF	Front Genet; Identification and Validation of Chromobox Family Members as Potential Prognostic Biomarkers and Therapeutic Targets for Human Esophageal Cancer.; Authors - Xuefen Fang
human	WB,IHC,IP	Adv Sci (Weinh); N^6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation; Authors - Yin Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Band at predicted size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart