Anticorps Polyclonal de lapin anti-CBX4

• Phare
• Validé par KD/KO

CBX4 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA, PLA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18544-1-AP

Synonymes

Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4

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Galerie de données de validation

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  WB analysis of HEK-293 cell using 18544-1-AP

  WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18544-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX4 antibody (18544-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: diverses cellules, cellule HEK-293, cellules PC-3
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

18544-1-AP cible CBX4 dans les applications de WB, IF/ICC, ELISA, PLA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX4 Protéine recombinante Ag13425
• Nom complet: chromobox homolog 4 (Pc class homolog, Drosophila)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: ~75 kDa
• Numéro d’acquisition GenBank: BC014967
• Symbole du gène: CBX4
• Identification du gène (NCBI): 8535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Publications

Species	Application	Title
Human		Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB,IF,PLA	Front Cell Dev Biol; SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.; Authors - Immacolata Giordano
human	WB	Bioengineered; Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.; Authors - Zengxi Yang ; KD Validated
human	WB	Mol Oncol; CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells; Authors - Liwei Ren
		EMBO J; Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer; Authors - Ivana Paskov Škapik
human,mouse	WB,IF	Cardiovasc Drugs Ther; Macrophage CBX4 Potentiates Atherosclerosis by its SUMO E3 Ligase Activity; Authors - Zhenyu Zhao

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