Anticorps Polyclonal de lapin anti-CBX4

• Phare
• Validé par KD/KO

CBX4 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, PLA, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 18544-1-AP

Synonymes

CBX4, Chromobox protein homolog 4, E3 SUMO protein ligase CBX4, hPC2, NBP16, PC2, Polycomb 2 homolog

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Galerie de données de validation

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  WB analysis of HEK-293 cell using 18544-1-AP

  WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS cell using 18544-1-AP

  IF result of anti-CBX4 (18544-1-AP, 1:200) with U2OS cell, MeOH fixation. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  IF Staining of HeLa using 18544-1-AP

  Immunofluorescent analysis of HeLa cells using 18544-1-AP (CBX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: diverses cellules, cellule HEK-293, cellules PC-3
• Résultats positifs en IF: cellule U2OS, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

18544-1-AP cible CBX4 dans les applications de WB, PLA, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX4 Protéine recombinante Ag13425
• Nom complet: chromobox homolog 4 (Pc class homolog, Drosophila)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: ~75 kDa
• Numéro d’acquisition GenBank: BC014967
• Symbole du gène: CBX4
• Identification du gène (NCBI): 8535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Publications

Species	Application	Title
human	WB,IF,PLA	Front Cell Dev Biol; SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.; Authors - Immacolata Giordano
human	WB	Bioengineered; Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.; Authors - Zengxi Yang ; KD Validated
human	WB	Mol Oncol; CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells; Authors - Liwei Ren