Anticorps Polyclonal de lapin anti-CBX6

CBX6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 23426-1-AP

Synonymes

CBX6, chromobox homolog 6, Chromobox protein homolog 6

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Galerie de données de validation

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  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

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  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

23426-1-AP cible CBX6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX6 Protéine recombinante Ag20014
• Nom complet: chromobox homolog 6
• Masse moléculaire calculée: 412 aa, 44 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC064900
• Symbole du gène: CBX6
• Identification du gène (NCBI): 23466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, involves in transcriptional repression, cell cycle regulation and chromatin remodeling [PMID: 17486619]. Cbx6, other named neuronal pentraxin receptor (NPR), first described as a putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2, has also been classified as a Pc protein [PMID: 14647293]. NPR was determined to be a member of a distinct pentraxin protein subfamily, and found to form heteropentamer swithNP1 andNP2, followed by release from cell membranes [PMID: 10748068].

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang