Anticorps Polyclonal de lapin anti-CBY1

CBY1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 12239-1-AP

Synonymes

arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1

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Galerie de données de validation

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  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12239-1-AP

  IP Result of anti-CBY1 (IP:12239-1-AP, 4ug; Detection:12239-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12239-1-AP cible CBY1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBY1 Protéine recombinante Ag2882
• Nom complet: chibby homolog 1 (Drosophila)
• Masse moléculaire calculée: 126 aa, 14 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC016139
• Symbole du gène: CBY1
• Identification du gène (NCBI): 25776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo
human	IHC	Medicine (Baltimore); Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma; Authors - Gang Ren

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Feng-Qian (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T