Anticorps Polyclonal de lapin anti-MCU/CCDC109A

• Phare
• Validé par KD/KO

MCU/CCDC109A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 26312-1-AP

Synonymes

C10orf42, Calcium uniporter protein, CCDC109A, MCU, MCU/CCDC109A

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Galerie de données de validation

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26312-1-AP

  WB result of MCU/CCDC109A antibody (26312-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCU/CCDC109A transfected HeLa cells.

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  IHC staining of mouse kidney using 26312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

26312-1-AP cible MCU/CCDC109A dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCU/CCDC109A Protéine recombinante Ag24733
• Nom complet: coiled-coil domain containing 109A
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC034235
• Symbole du gène: CCDC109A
• Identification du gène (NCBI): 90550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).

Publications

Species	Application	Title
human	WB	Cell Death Differ; Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak.; Authors - Giovanni Quarato
human	WB	Environ Pollut; TDCPP and TiO2 NPs aggregates synergistically induce SH-SY5Y cell neurotoxicity by excessive mitochondrial fission and mitophagy inhibition; Authors - Ling Wang
human	WB	Cell Rep; A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease; Authors - Vinita Bharat
mouse	WB	Int J Mol Sci; Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes; Authors - Tianyang Lan
mouse	WB	J Agric Food Chem; Puerarin Ameliorated PCOS through Preventing Mitochondrial Dysfunction Dependent on the Maintenance of Intracellular Calcium Homeostasis; Authors - Yu-Si Wang
mouse	WB	iScience; Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage; Authors - Tianyou Kan

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