Anticorps Polyclonal de lapin anti-CCDC111/PRIMPOL

• Phare
• Validé par KD/KO

CCDC111/PRIMPOL Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 29824-1-AP

Synonymes

CCDC111, FLJ33167, hPrimpol1, PRIMPOL

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Galerie de données de validation

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  WB analysis using 29824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 29824-1-AP

  WB result of CCDC111/PRIMPOL antibody (29824-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC111/PRIMPOL transfected HepG2 cells.

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  IHC staining of human kidney using 29824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 29824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC111 antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 29824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC111 antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

29824-1-AP cible CCDC111/PRIMPOL dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC111/PRIMPOL Protéine recombinante Ag31478
• Nom complet: coiled-coil domain containing 111
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC064600
• Symbole du gène: CCDC111
• Identification du gène (NCBI): 201973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

Publications

Species	Application	Title
human	WB	Nat Commun; An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress; Authors - Taichi Igarashi
		Nat Commun; Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases; Authors - Anastasia Hale
human	WB	Int J Mol Sci; CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin; Authors - Nicole L Batenburg

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hasan (Verified Customer) (01-31-2023)

WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa cells/ different clones

CCDC111/PRIMPOL Antibody Western Blot validation (1:2000 dilution) in HeLa cells/ different clones (Cat no:29824-1-AP)