• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-CCDC111/PRIMPOL

CCDC111/PRIMPOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 29824-1-AP

Synonymes

CCDC111, FLJ33167, hPrimpol1, PRIMPOL



Applications testées

Résultats positifs en WBcellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
Résultats positifs en IHCtissu rénal humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules U2OS,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

29824-1-AP cible CCDC111/PRIMPOL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CCDC111/PRIMPOL Protéine recombinante Ag31478
Nom complet coiled-coil domain containing 111
Poids moléculaire observé64 kDa
Numéro d’acquisition GenBankBC064600
Symbole du gène CCDC111/PRIMPOL
Identification du gène (NCBI) 201973
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

Protocole

Product Specific Protocols
WB protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IHC protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IF protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress

Authors - Taichi Igarashi

Nat Commun

Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases

Authors - Anastasia Hale
humanWB

Int J Mol Sci

CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin

Authors - Nicole L Batenburg
humanWB

bioRxiv

CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps

Authors - Lindsey M Pale
humanWB

Nucleic Acids Res

USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress

Authors - Alexandra Nusawardhana
humanWB

Cell Rep

FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition

Authors - Zeyuan Liu
  • KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hasan (Verified Customer) (01-31-2023)

WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa cells/ different clones
CCDC111/PRIMPOL Antibody Western Blot validation (1:2000 dilution) in HeLa cells/ different clones (Cat no:29824-1-AP)
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