- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CCDC111/PRIMPOL
CCDC111/PRIMPOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 29824-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat |
| Résultats positifs en IHC | tissu rénal humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules U2OS, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 4 publications below |
| WB | See 15 publications below |
| IF | See 1 publications below |
Informations sur le produit
29824-1-AP cible CCDC111/PRIMPOL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CCDC111/PRIMPOL Protéine recombinante Ag31478 |
| Nom complet | coiled-coil domain containing 111 |
| Poids moléculaire observé | 64 kDa |
| Numéro d’acquisition GenBank | BC064600 |
| Symbole du gène | CCDC111/PRIMPOL |
| Identification du gène (NCBI) | 201973 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
| IHC protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
| IF protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress | ||
Nat Commun Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases | ||
Int J Mol Sci CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin | ||
bioRxiv CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps | ||
Nucleic Acids Res USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress | ||
Cell Rep FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hasan (Verified Customer) (01-31-2023) | WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.
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