Anticorps Monoclonal anti-CEP89, CCDC123

CEP89, CCDC123 Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1F12C5

N° de cat : 68112-1-Ig

Synonymes

CEP89, CCDC123, 1F12C5, Cep123, Centrosomal protein of 89 kDa

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Galerie de données de validation

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  WB analysis of Neuro-2a using 68112-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CEP89, CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of hTERT-RPE1 using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules Neuro-2a, cellules SH-SY5Y, cellules U-251
• Résultats positifs en IF-P: tissu oculaire de souris, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68112-1-Ig cible CEP89, CCDC123 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CEP89, CCDC123 Protéine recombinante Ag28339
• Nom complet: coiled-coil domain containing 123
• Masse moléculaire calculée: 783 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC136328
• Symbole du gène: CEP89
• Identification du gène (NCBI): 84902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).

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