Anticorps Polyclonal de lapin anti-CCDC124

CCDC124 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27106-1-AP

Synonymes

CCDC124

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Galerie de données de validation

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  WB analysis of mouse brain using 27106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27106-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27106-1-AP cible CCDC124 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC124 Protéine recombinante Ag25930
• Nom complet: coiled-coil domain containing 124
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC013949
• Symbole du gène: CCDC124
• Identification du gène (NCBI): 115098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.

Publications

Species	Application	Title
human	WB,IF	Protein J; Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in G3BP1-Induced Stress-Granules; Authors - Kübra Hacibeyoğlu

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