Anticorps Polyclonal de lapin anti-CCDC22

• Phare
• Validé par KD/KO

CCDC22 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(20)

N° de cat : 16636-1-AP

Synonymes

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

---

Galerie de données de validation

• 

  WB analysis of human brain using 16636-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16636-1-AP

  WB result of CCDC22 antibody (16636-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.

• 

  WB analysis of rat colon using 16636-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16636-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16636-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16636-1-AP

  IP result of anti-CCDC22 (IP:16636-1-AP, 4ug; Detection:16636-1-AP 1:600) with mouse lung tissue lysate 3600ug.

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, tissu de côlon de rat, tissu de côlon de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

16636-1-AP cible CCDC22 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC22 Protéine recombinante Ag9980
• Nom complet: coiled-coil domain containing 22
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC000972
• Symbole du gène: CCDC22
• Identification du gène (NCBI): 28952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi
human	WB, IP	J Cell Biol; Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.; Authors - Haiying Li
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang

{{ptg:RelatedPrimaryAntibodies}}