Anticorps Polyclonal de lapin anti-CCDC65

CCDC65 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 24376-1-AP

Synonymes

Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65

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Galerie de données de validation

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  WB analysis of mouse testis using 24376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 24376-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24376-1-AP

  IP result of anti-CCDC65 (IP:24376-1-AP, 4ug; Detection:24376-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of U2OS using 24376-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCDC65 antibody (24376-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24376-1-AP cible CCDC65 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC65 Protéine recombinante Ag19357
• Nom complet: coiled-coil domain containing 65
• Masse moléculaire calculée: 484 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC039317
• Symbole du gène: CCDC65
• Identification du gène (NCBI): 85478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.; Authors - Ziyan Zhang
human	IF	Andrology; A recurrent loss-of-function variant in DRC1 causes non-syndromic severe asthenozoospermia with favorable intracytoplasmic sperm injection and pregnancy outcomes; Authors - Célia Tebbakh

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