Anticorps Polyclonal de lapin anti-CCDC93

• Phare
• Validé par KD/KO

CCDC93 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 20861-1-AP

Synonymes

CCDC93

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Galerie de données de validation

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  WB analysis using 20861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20861-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20861-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 20861-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20861-1-AP

  IP result of anti-CCDC93 (IP:20861-1-AP, 4ug; Detection:20861-1-AP 1:500) with L02 cells lysate 1800ug.

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  IHC staining of human colon using 20861-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20861-1-AP (CCDC93 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20861-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20861-1-AP (CCDC93 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, tissu de côlon de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

20861-1-AP cible CCDC93 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC93 Protéine recombinante Ag14855
• Nom complet: coiled-coil domain containing 93
• Masse moléculaire calculée: 631 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC028609
• Symbole du gène: CCDC93
• Identification du gène (NCBI): 54520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
mouse	WB	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi
human	WB	J Cell Biol; Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.; Authors - Haiying Li
human	WB,IF	Elife; Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.; Authors - Sai Srinivas Panapakkam Giridharan
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang
human	CoIP	Mol Cancer Res; COMMD3-mediated endosomal trafficking of HER2 inhibits the progression of ovarian carcinoma; Authors - Shiqing Wang

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