Anticorps Polyclonal de lapin anti-CCKBR-specific

CCKBR-specific Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16549-1-AP

Synonymes

CCK B, CCK B receptor, CCK BR, CCK2 R, CCK2R, CCKBR, CCKRB, Cholecystokinin 2 receptor, cholecystokinin B receptor, GASR

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Galerie de données de validation

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  WB analysis of BxPC-3 using 16549-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 16549-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 16549-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 16549-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules SGC-7901, tissu d'estomac humain
• Résultats positifs en IF: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16549-1-AP cible CCKBR-specific dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cholecystokinin B receptor
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 64 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_176875
• Symbole du gène: CCKBR
• Identification du gène (NCBI): 887
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Publications

Species	Application	Title
human	WB,IHC	Mol Pharm; Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.; Authors - Jiang Chang
human	IF	J Cancer Res Clin Oncol; Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.; Authors - Jiang Chang

Produits associés

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11623-1-AP