Anticorps Recombinant de lapin anti-CCL12/MCP-5

CCL12/MCP-5 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250386G10

N° de cat : 98542-1-RR

Synonymes

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells and CCL12/MCP-5-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse Ccl12 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells (left) or CCL12/MCP-5-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL12/MCP-5 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98542-1-RR cible CCL12/MCP-5 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL12/MCP-5 Protéine recombinante Eg3315
• Nom complet: chemokine (C-C motif) ligand 12
• Masse moléculaire calculée: 12KD
• Numéro d’acquisition GenBank: NM_011331.2
• Symbole du gène: Ccl12
• Identification du gène (NCBI): 20293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.

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