Anticorps Recombinant de lapin anti-CCL20/MIP-3 alpha

CCL20/MIP-3 alpha Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250239E12

N° de cat : 98519-1-RR

Synonymes

Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98519-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98519-1-RR against Mouse CCL20/MIP-3 alpha were performed. The affinity constant is 3.09 nM.

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98519-1-RR cible CCL20/MIP-3 alpha dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL20/MIP-3 alpha Protéine recombinante Eg3311
• Nom complet: chemokine (C-C motif) ligand 20
• Masse moléculaire calculée: 11KD
• Numéro d’acquisition GenBank: NM_001159738.1
• Symbole du gène: Ccl20
• Identification du gène (NCBI): 20297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

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