Anticorps Recombinant de lapin anti-CCL22/MDC

CCL22/MDC Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250115D3

N° de cat : 98501-2-RR

Synonymes

Abcd1, Activated B and dendritic cell-derived, CC chemokine ABCD-1, C-C motif chemokine 22, Ccl22

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98501-2-RR

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98501-2-RR

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98501-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98501-2-RR against Mouse CCL22/MDC were performed. The affinity constant is 0.914 nM.

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98501-2-RR cible CCL22/MDC dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL22/MDC Protéine recombinante Eg2241
• Nom complet: chemokine (C-C motif) ligand 22
• Masse moléculaire calculée: 10kd
• Numéro d’acquisition GenBank: NM_009137
• Symbole du gène: CCL22/MDC
• Identification du gène (NCBI): 20299
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.

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