Anticorps Recombinant de lapin anti-CCL9

CCL9 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

243103H5

N° de cat : 98475-1-RR

Synonymes

C-C motif chemokine 9, CCF18, CCL9(29-101), CCL9(30-101), CCL9(31-101)

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98475-1-RR

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone: 243103H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98475-1-RR

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone:243103H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98475-1-RR against Mouse CCL9 were performed. The affinity constant is 0.649 nM.

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98475-1-RR cible CCL9 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL9 Protéine recombinante Eg3336
• Nom complet: chemokine (C-C motif) ligand 9
• Masse moléculaire calculée: 14KD
• Numéro d’acquisition GenBank: NM_011338.2
• Symbole du gène: Ccl9
• Identification du gène (NCBI): 20308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Mouse C-C motif ligand 9 (CCL9), alternatively named macrophage inflammatory protein 1γ (MIP-1γ), was identified in 1995 and is homologous to the mouse CCL6, as well as human CCL23 and CCL15. It is noted that CCL9 is also known by various names such as macrophage inflammatory protein-1 gamma (MIP-1ɣ), macrophage inflammatory protein-related protein-2 (MRP-2) and CCF18 in rodents. Monocytes and myeloid cell lines produce large quantities of CCL9, as do dendritic cells and T cells, in particular Th1 type T cells. Despite high baseline levels in circulation, it has become apparent that concentrations of CCL9 vary greatly in specific tissues with profound effects on health. For example, in the bone, CCL9 is produced at even higher levels, and is critical to osteoclast versus osteoblast differentiation of macrophages. There are also indications of a timed specific induction of CCL9 in skin wound healing and follicle-associated epithelium of the gut.

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