Anticorps Polyclonal de lapin anti-Cyclin B2

• Phare
• Validé par KD/KO

Cyclin B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 21644-1-AP

Synonymes

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

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Galerie de données de validation

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  WB analysis using 21644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21644-1-AP

  WB result of Cyclin B2 antibody (21644-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected A431 cells.

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  WB analysis of HeLa using 21644-1-AP

  Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 21644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 21644-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 21644-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 21644-1-AP

  IP Result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.

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  IHC staining of human cervical cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules DU 145, cellules HeLa, cellules K-562, tissu testiculaire de rat
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

21644-1-AP cible Cyclin B2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin B2 Protéine recombinante Ag16329
• Nom complet: cyclin B2
• Masse moléculaire calculée: 398 aa, 45 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC105086
• Symbole du gène: CCNB2
• Identification du gène (NCBI): 9133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. Cyclin B2 can be detected double bands (PMID:1651338).

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling; Authors - Chaojie Chen
human	WB,IP	EMBO Rep; Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.; Authors - Sikai Liu ; KD Validated
human	WB	Oxid Med Cell Longev; Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.; Authors - Mei Cao
human	IHC	Elife; Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.; Authors - Xiao-Tong Lin
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
human	WB	Ecotoxicol Environ Saf; Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.; Authors - Liling Su