Anticorps Monoclonal anti-Cyclin D1

• Phare
• Validé par KD/KO

Cyclin D1 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(824)

CloneNo.

2G3G5

Avis (3)

N° de cat : 60186-1-Ig

Synonymes

CCND1, 2G3G5, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1

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Galerie de données de validation

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  WB analysis using 60186-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60186-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60186-1-Ig

  WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells.

• 

  WB analysis using 60186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60186-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60186-1-Ig

  Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 60186-1-Ig

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Cyclin D1 Monoclonal antibody (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules A549, cellules HCT 116, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, cellules SW-1990, cellules U2OS
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 796 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• IP: See 4 publications below

Informations sur le produit

60186-1-Ig cible Cyclin D1 dans les applications de WB, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, Lapin, poisson-zèbre, poulet, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin D1 Protéine recombinante Ag0689
• Nom complet: cyclin D1
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000076
• Symbole du gène: Cyclin D1
• Identification du gène (NCBI): 595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.; Authors - Guixin Zhu
human	WB	Bioact Mater; Bioinspired engineering ADSC nanovesicles thermosensitive hydrogel enhance autophagy of dermal papilla cells for androgenetic alopecia treatment; Authors - Jiachao Xiong
human	IP	Nat Commun; F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression; Authors - Feng Li
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
mouse	WB	Adv Sci (Weinh); Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis; Authors - Bin Liu
mouse	WB	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Janardhan (Verified Customer) (08-22-2020)

Antibody works ok, needs longer exposure

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Medulloblastoma

FH

Yuan (Verified Customer) (01-23-2020)

The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hela cell

FH

Kyosuke (Verified Customer) (06-12-2019)

I use this as a proliferation marker and this antibody works very well for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: rat aortic smooth muscle cells

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