Anticorps Monoclonal anti-Cyclin D2

Cyclin D2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1A12A8

N° de cat : 67048-1-Ig

Synonymes

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002

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Galerie de données de validation

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  WB analysis using 67048-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67048-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Caco-2, cellules MCF-7, cellules NIH/3T3, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

67048-1-Ig cible Cyclin D2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin D2 Protéine recombinante Ag25190
• Nom complet: cyclin D2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC010958
• Symbole du gène: Cyclin D2
• Identification du gène (NCBI): 894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publications

Species	Application	Title
human	WB	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma
human	WB	Technol Cancer Res Treat; The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.; Authors - AiJun Huang
human	WB	Cell Death Dis; Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3.; Authors - Shuaishuai Zhuo
human,mouse	WB	Am J Cancer Res; miR-1297 inhibits osteosarcoma cell proliferation and growth by targeting CCND2; Authors - Pan Jiang
human	WB	Int Immunopharmacol; Hydroxypropyl-β-cyclodextrin inhibits the development of triple negative breast cancer by enhancing antitumor immunity; Authors - Mengmeng Zhu
human	WB	Cancer Chemother Pharmacol; Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway; Authors - Xiang Fei

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