Anticorps Polyclonal de lapin anti-Cyclin G

Cyclin G Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10897-1-AP

Synonymes

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 10897-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10897-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10897-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, cellules HeLa, tissu hépatique de souris, tissu ovarien de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

10897-1-AP cible Cyclin G dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin G Protéine recombinante Ag1157
• Nom complet: cyclin G1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC007093
• Symbole du gène: CCNG1
• Identification du gène (NCBI): 900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1.; Authors - Mingjing Yan
human	WB	Cancer Lett; The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1.; Authors - Chen-Song Huang
human	WB	J Biol Chem; MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.; Authors - Fanpeng Zhao
human	WB	Exp Cell Res; PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1; Authors - Yuzhong Chen
human	WB	Oncol Res; Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2; Authors - Yusen Zhang