Anticorps Polyclonal de lapin anti-CCPG1

• Phare
• Validé par KD/KO

CCPG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(21)

Avis (2)

N° de cat : 13861-1-AP

Synonymes

KIAA1254, CPR8, CCP8

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Galerie de données de validation

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  WB analysis using 13861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13861-1-AP (CCPG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13861-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13861-1-AP (CCPG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13861-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13861-1-AP (CCPG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13861-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCPG1 antibody (13861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cellules THP-1
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13861-1-AP cible CCPG1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCPG1 Protéine recombinante Ag4841
• Nom complet: cell cycle progression 1
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC034914
• Symbole du gène: CCPG1
• Identification du gène (NCBI): 9236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell-cycle progression gene 1 (CCPG1) is a receptor for ER stress-induced ER-phagy. CCPG1 localizes to perinuclear ER and in small foci at the ER periphery. It contains a LIR at the N-terminal cytosolic domain engaging LC3/GABARAP. (PMID: 29802216, PMID: 29290589)

Publications

Species	Application	Title
human	WB	Nat Commun; AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity; Authors - Linliang Zhang
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner
human	WB	Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham ; KD Validated
rat	WB	J Pineal Res; Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression; Authors - Min Yao
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
human	WB,IP	Dev Cell; CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.; Authors - Matthew D Smith ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhe (Verified Customer) (09-12-2025)

There are several nonspecific bands showing up between 40-70kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, liver

FH

H (Verified Customer) (04-05-2020)

This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

CCPG1 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:13861-1-AP)

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