Anticorps Polyclonal de lapin anti-CCR2a-specific

• Phare
• Validé par KD/KO

CCR2a-specific Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (3)

N° de cat : 16153-1-AP

Synonymes

C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R

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Galerie de données de validation

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  WB analysis of Jurkat using 16153-1-AP

  WB result of CCR2a-specific antibody (16153-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCR2a-specific transfected Jurkat cells.

• 

  WB analysis using 16153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16153-1-AP (CCR2a-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  FC experiment of Jurkat using 16153-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug CCR2-specific antibody (16153-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

16153-1-AP cible CCR2a-specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chemokine (C-C motif) receptor 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_000647
• Symbole du gène: CCR2
• Identification du gène (NCBI): 729230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.

Publications

Species	Application	Title
human	WB	Lab Invest; Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.; Authors - Shin Ung Kang
human	WB	Oncol Rep; CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.; Authors - Huijie Zhuang
rat	WB	J Ethnopharmacol; Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis; Authors - Tian Xu
mouse	WB,IF	Mol Immunol; QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.; Authors - Lele Deng
human,rat	WB,IHC	J Physiol Biochem; Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues; Authors - Bairong Fang
human	IHC	Clin Exp Rheumatol; Curcumin alleviates inflammation in Takayasu's arteritis by blocking CCL2 overexpression in adventitial fibroblasts.; Authors - Sifan Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal Baral (Verified Customer) (01-26-2024)

Bone marrow mesenchymal stem cells from mouse stained great at the provided dilution.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse bone marrow

FH

Kamal (Verified Customer) (01-26-2024)

Bands appeared at 35 kDa. Antibody works well when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

Alexandru (Verified Customer) (11-13-2023)

Great stainig results on paraffin-embedded tissue!

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

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