Anticorps Monoclonal anti-CCR6

CCR6 Monoclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1C11A9

N° de cat : 66801-1-Ig

Synonymes

1C11A9, BN 1, C C chemokine receptor type 6, C C CKR 6, C-C chemokine receptor type 6

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Galerie de données de validation

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  IHC staining of human tonsillitis using 66801-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66801-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66801-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Jurkat using 66801-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66801-1-Ig cible CCR6 dans les applications de IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCR6 Protéine recombinante Ag24195
• Nom complet: chemokine (C-C motif) receptor 6
• Masse moléculaire calculée: 42 kDa
• Numéro d’acquisition GenBank: BC037960
• Symbole du gène: CCR6
• Identification du gène (NCBI): 1235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCR6, also known as CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed in most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells, and B-cells and plays a role in skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).

Publications

Species	Application	Title
human	IHC	Neoplasia; Gut microbiota-derived short-chain fatty acids promote prostate cancer progression via inducing cancer cell autophagy and M2 macrophage polarization; Authors - Yufei Liu
	IF	Cell Death Dis; RANKL/RANK signaling recruits Tregs via the CCL20-CCR6 pathway and promotes stemness and metastasis in colorectal cancer; Authors - Jing Ouyang
human	IF	Clin Immunol; Exploring TH17-mediated inflammation in epidermolytic ichthyosis: Clinical and mechanistic insight; Authors - Yidong Tan

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